First frame defaults to 1 and Last frame defaults to the number of t-frames). The default behavior is to include all t-frames (i.e. First frame is the 1-based offset of the first t-frame to include in the resulting image Last frame is the 1-based index of the last t-frame to include. First frame/Last frame Those settings allow to create an image containing only a subset of the t-frames from the original hyperstack. Settings in this section are only meaningful for hyperstacks containing several time-frames (t-frames). If this option is not enabled, the overlay will be duplicated as needed to be carried over to all panels. Copy original overlay only once If the original hyperstack contains an overlay and this option is enabled, the overlay will only be carried over to the first panel (leftmost panel or top panel, depending on the orientation setting) of the final image. Font size This is the size of the font used to display the labels. Separator width If this option is enabled, the panels will be separated by a white line whose width (in pixels) can also be specified. This section contains the following settings: Orientation This defines whether the panels will be placed next to each other, with the merged panel on the right ( Horizontal), or one on top of another, with the merged panel at the bottom Vertical). Settings dialog for the Unified_Merge plugin General settings The settings are organized in several sections. When the plugin is called, you´re presented with a settings dialog lile the one depicted below.
From that source, the plugin generated an image composed of four panels side by side: the three leftmost panels depict the individual channels, in grayscale the rightmost panel depicts a composite colored image of all three channels merged together. The hyperstack used as the source image was made of three different channels (DiC, mCherry::H2A.Z, and GFP::Polo). To illustrate the purpose of this plugin, let´s consider a sample output in the figure above. Sample output from the Unified_Merge plugin This plugin allows to create an image which may contain one or several individual channels in grayscale alongside a colored merge of several channels.
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